INTEGRATED CONFOCAL MICROSCOPE USING PLANAR OPTICS

S. Sinzinger, J. Jahns
Optische Nachrichtentechnik

Confocal microscopy is an imaging technique widely used in scanning microscopes, for sensing, and optical data storage. A point source is used in combination with a point detector to yield enhanced lateral resolution as well as depth discrimination [1]. In the conventional configuration the point source is imaged onto the object by an objective lens. Light reflected or scattered from the illuminated object point is collected by the same objective lens and imaged via a beam splitter onto the detector. The detector intensity is influenced by changes of the reflectivity R of the illuminated object point x_s . A thickness variation of the object results in defocusing and a sharp drop of the detector intensity I_D . With the numerical aperture NA of the objective lenses and the defocusing delta z , I_D can be approximated as:

Formel1

If assembled with conventional optical components and optomechanical alignment a bulky setup results. In this case size and stability problems limit the variety of applications to which the confocal concept can be applied. Such problems can be overcome with an integrated version of the confocal microscope.

Planar optics is an approach to the integration of free-space optical setups and thus can be applied to the integration of confocal microscopes [2]. To this end all necessary components are integrated on the surfaces of a glass substrate. Fig. 1 shows the schematic of a fully integrated confocal sensor head. The optical system consists of two collimating microlenses (L1 and L4) and two off-axis lens segments (L2 and L3) integrated on opposite sites of a glass substrate. The optical axis is folded into an off-axis configuration typical for planar optics. The depth discrimination now depends on both the aperture angle beta of the optical system as well as the off axis angle alpha :

Formel2

This approach leads to a compact, stable, small size, and light-weight sensor head. The planar optical setup offers high functionality and the potential to build fully integrated systems including optoelectronic and electronic components through hybrid integration techniques.

confocal mikroscope

Fig. 1: Integrated confocal sensor head

References:

[1] T. Wilson, C. Sheppard, "Theory and practice of scanning optical microscopy", Academic Press, London (1984).

[2] S. Sinzinger, J. Jahns, "Integrated confocal microscope based on planar optics", OSA Annual Meeting, Portland (1995), paper ThRR7.

zurück zur Übersicht "Jahresbericht 1995"